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anti il6 af 206 na antibodies  (R&D Systems)


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    Structured Review

    R&D Systems anti il6 af 206 na antibodies
    Anti Il6 Af 206 Na Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il6/Human+IL-6+Antibody/pm41997110-259-36-40
    Average 93 stars, based on 77 article reviews
    anti il6 af 206 na antibodies - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Telomerase modRNA offers a novel RNA-based approach to treat human pulmonary fibrosis
    Article Snippet: PCLS culture supernatants were collected after 96 h, supplemented with 0.2 % protease inhibitor cocktail (87785 Thermo Fisher Scientific), and stored at −80 °C. .. Human IL6, IL8, TGFβ and pro-COL1A1 was measured using DuoSet ELISA kits from R&D Systems (DY206, DY208, DY6220 and DY240) according to the manufacturer’s recommendations. .. Senescence-associated β-galactosidase (SA-β-gal) levels were assessed within the tissue after 96 h using a β-Gal Staining Kit (9860 Cell Signalling) and performed according to the manufacturer’s recommendations adjusting staining/fixation volumes to 500 μL per PCLS.

    Article Title: Telomerase modRNA Offers a Novel RNA-Based Approach to Treat Human Pulmonary Fibrosis.
    Article Snippet: PCLS culture supernatants were collected after 96 h, supplemented with 0.2% protease inhibitor cocktail (87,785 Thermo Fisher Scientific), and stored at −80°C. .. Human IL6, IL8, TGFβ and pro- COL1A1 were measured using DuoSet ELISA kits from R&D Systems (DY206, DY208, DY6220 and DY240) according to the manufacturer's recommendations. .. Broad panel of immune cytokines was measured using V- PLEX Pro- inflammatory Panel 1 Human Kit (K15049D, Meso Scale Diagnostics) according to manufacturer's recommendations.

    Article Title: The Scap-SREBP1-S1P/S2P lipogenesis signal orchestrates the homeostasis and spatiotemporal activation of NF-κB.
    Article Snippet: .. The secreted protein levels of human IL6 (D6050, R&D), IL8(D8000C, R&D), mouse IL6 (M6000B, R&D), CXCL1 (MKC00B, R&D), and MIP2 (MM200, R&D) in cell culture supernatants or bronchoalveolar lavage fluid (BALF) supernatants were determined with ELISA kits following the manufacturer’s protocols. ..

    Article Title: hESC-derived extracellular vesicles enriched with MFGE-8 and the GSH redox system act as senotherapeutics for neural stem cells in ischemic stroke.
    Article Snippet: Human embryonic stem cells (hESCs) and their extracellular vesicles (EVs) hold significant potential for tissue repair and regeneration.. Neural stem cells (NSCs) in the adult brain often acquire senescent phenotypes after ischemic injuries, releasing neurodegenerative senescence-associated secretory phenotype factors.. In this study, we investigated the senotherapeutic effects of hESC-EVs on NSCs and confirmed their neuroprotective effects in neurons via rejuvenation of NSC secretions.

    Cell Culture:

    Article Title: The Scap-SREBP1-S1P/S2P lipogenesis signal orchestrates the homeostasis and spatiotemporal activation of NF-κB.
    Article Snippet: .. The secreted protein levels of human IL6 (D6050, R&D), IL8(D8000C, R&D), mouse IL6 (M6000B, R&D), CXCL1 (MKC00B, R&D), and MIP2 (MM200, R&D) in cell culture supernatants or bronchoalveolar lavage fluid (BALF) supernatants were determined with ELISA kits following the manufacturer’s protocols. ..

    Article Title: Innovative evaluation of selinexor and JQ1 synergy in leukemia therapy via C-MYC inhibition
    Article Snippet: Mononuclear cells were separated from BM samples by LymphoprepTM (STEMCELL Technologies, Canada) using density gradient certification. .. The mononuclear cells were cultured in StemSpanTM SFEM II medium (STEMCELL Technologies, Canada) supplemented with 10 ng/mL rh-SCF, 10 ng/mL human IL3, 10 ng/mL human IL6 (all cytokines were purchased from R&D Systems, USA). ..

    Recombinant:

    Article Title: Proteolytic profiling of human plasma reveals an immunoactive complement C3 fragment.
    Article Snippet: .. Incubations were performed with 100 μL of fresh media, supplemented with recombinant human IL6 (R&D Systems #7270- IL/CF) as a positive control, reconstituted in 1x PBS, or C3-LHF1 o/N at 37 °C, 5% CO2. ..

    Article Title: Human Recombinant Interleukin-6 and Leukemia Inhibitory Factor Improve Inner Cell Mass Cell Number but Lack Cryoprotective Activities on In Vitro-Produced Bovine Blastocysts
    Article Snippet: .. Concentrated stocks of recombinant human IL6, IL11, and LIF (10 μg/mL; R&D Systems, Minneapolis, MN, USA) were prepared in SOF base stock solution with 1% [ w / v ] bovine serum albumin (BSA). ..

    Article Title: Proteolytic profiling of human plasma reveals an immunoactive complement C3 fragment
    Article Snippet: .. Incubations were performed with 100 μL of fresh media, supplemented with recombinant human IL6 (R&D Systems #7270-IL/CF) as a positive control, reconstituted in 1x PBS, or C3-LHF1 o/N at 37 °C, 5% CO 2 . ..

    Positive Control:

    Article Title: Proteolytic profiling of human plasma reveals an immunoactive complement C3 fragment.
    Article Snippet: .. Incubations were performed with 100 μL of fresh media, supplemented with recombinant human IL6 (R&D Systems #7270- IL/CF) as a positive control, reconstituted in 1x PBS, or C3-LHF1 o/N at 37 °C, 5% CO2. ..

    Article Title: Proteolytic profiling of human plasma reveals an immunoactive complement C3 fragment
    Article Snippet: .. Incubations were performed with 100 μL of fresh media, supplemented with recombinant human IL6 (R&D Systems #7270-IL/CF) as a positive control, reconstituted in 1x PBS, or C3-LHF1 o/N at 37 °C, 5% CO 2 . ..



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    Tumor-associated inflammation promotes the expression of LRG1 in hepatocytes by <t>IL6/STAT3.</t> A The relative expression of Lrg1 in AML12 cells co-cultured with various mouse tumor cell lines or treated with serum (5%) from CRC model mice. n = 3 independent experiments. Data are means ± SD. B Western blot analysis of the expression LRG1 and β-actin in AML12 cells after the indicated treatments. C Cytokine array analyses of serum from CRC orthotopic mice model (day 7, day 14, day 21 and day 28) and from CRC intrasplenic mice model (day 5, day 10, day 15 and day 21). Quantitative real-time PCR analyses of the expression Lrg1 D and ELISA analyses of media of AML12 E treated with vehicle or recombinant IL6/G-CSF/CXCL13/CCL12/TIMP1. n = 3 independent experiments. Data are means ± SD. F, G qRT-PCR and Western blot analyses of LRG1 in AML12 cells treated with cytokine combinations. n = 3 independent experiments. Data are means ± SD. H, I qRT-PCR and western blot analysis of the expression LRG1 and β-actin in AML12 cells treated with indicated serum and anti-IL6 or tocilizumab. n = 3 independent experiments. Data are means ± SD. J ELISA analyses of serum samples for IL6 from sham-group, CRC orthotopic model at day 21(PMN) and CRC intrasplenic model at day 21(Met) in BALB/c. n = 4. Data are means ± SD. K Correlation between serological IL6 and LRG1 levels of CRC patients is shown using Pearson’s correlation analysis. Dots represent individual samples. n = 161. L, M Schematic of the experimental design L . Representative images of liver metastases M in each group ( n = 6 in Lrg1 (+/+)Hep-HTVi-Ctrl group and Lrg1 (+/+)Hep-HTVi-IL6 group, n = 5 in Lrg1 (Δ/Δ)Hep-HTVi-Ctrl group and Lrg1 (Δ/Δ)Hep-HTVi-IL6 group). Scale bars, 1 cm. N Schematic of the experimental design. Representative bioluminescence images and analyses of liver metastases in CRC mouse model treated with anti-IL6 or IgG. O Schematic of the experimental design. P ELISA analyses of samples for IL6 levels from liver interstitial fluid, peripheral blood and tumor interstitial fluid of mice from different groups as indicated. n = 4. Data are means ± SD. Q ELISA analyses of samples for LRG1 levels from liver interstitial fluid and peripheral blood of mice from different groups as indicated. n = 4. Data are means ± SD. R Western blot analyses of LRG1 and β-actin in hepatocyte of mice from different groups as indicated. Statistical significance was determined using two-tailed unpaired Student’s t test A, D–F, H, and N–P
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    Image Search Results


    Tumor-associated inflammation promotes the expression of LRG1 in hepatocytes by IL6/STAT3. A The relative expression of Lrg1 in AML12 cells co-cultured with various mouse tumor cell lines or treated with serum (5%) from CRC model mice. n = 3 independent experiments. Data are means ± SD. B Western blot analysis of the expression LRG1 and β-actin in AML12 cells after the indicated treatments. C Cytokine array analyses of serum from CRC orthotopic mice model (day 7, day 14, day 21 and day 28) and from CRC intrasplenic mice model (day 5, day 10, day 15 and day 21). Quantitative real-time PCR analyses of the expression Lrg1 D and ELISA analyses of media of AML12 E treated with vehicle or recombinant IL6/G-CSF/CXCL13/CCL12/TIMP1. n = 3 independent experiments. Data are means ± SD. F, G qRT-PCR and Western blot analyses of LRG1 in AML12 cells treated with cytokine combinations. n = 3 independent experiments. Data are means ± SD. H, I qRT-PCR and western blot analysis of the expression LRG1 and β-actin in AML12 cells treated with indicated serum and anti-IL6 or tocilizumab. n = 3 independent experiments. Data are means ± SD. J ELISA analyses of serum samples for IL6 from sham-group, CRC orthotopic model at day 21(PMN) and CRC intrasplenic model at day 21(Met) in BALB/c. n = 4. Data are means ± SD. K Correlation between serological IL6 and LRG1 levels of CRC patients is shown using Pearson’s correlation analysis. Dots represent individual samples. n = 161. L, M Schematic of the experimental design L . Representative images of liver metastases M in each group ( n = 6 in Lrg1 (+/+)Hep-HTVi-Ctrl group and Lrg1 (+/+)Hep-HTVi-IL6 group, n = 5 in Lrg1 (Δ/Δ)Hep-HTVi-Ctrl group and Lrg1 (Δ/Δ)Hep-HTVi-IL6 group). Scale bars, 1 cm. N Schematic of the experimental design. Representative bioluminescence images and analyses of liver metastases in CRC mouse model treated with anti-IL6 or IgG. O Schematic of the experimental design. P ELISA analyses of samples for IL6 levels from liver interstitial fluid, peripheral blood and tumor interstitial fluid of mice from different groups as indicated. n = 4. Data are means ± SD. Q ELISA analyses of samples for LRG1 levels from liver interstitial fluid and peripheral blood of mice from different groups as indicated. n = 4. Data are means ± SD. R Western blot analyses of LRG1 and β-actin in hepatocyte of mice from different groups as indicated. Statistical significance was determined using two-tailed unpaired Student’s t test A, D–F, H, and N–P

    Journal: Cellular and Molecular Immunology

    Article Title: Hepatocyte-derived LRG1 primes the liver for metastasis and impairs immunotherapy

    doi: 10.1038/s41423-026-01408-9

    Figure Lengend Snippet: Tumor-associated inflammation promotes the expression of LRG1 in hepatocytes by IL6/STAT3. A The relative expression of Lrg1 in AML12 cells co-cultured with various mouse tumor cell lines or treated with serum (5%) from CRC model mice. n = 3 independent experiments. Data are means ± SD. B Western blot analysis of the expression LRG1 and β-actin in AML12 cells after the indicated treatments. C Cytokine array analyses of serum from CRC orthotopic mice model (day 7, day 14, day 21 and day 28) and from CRC intrasplenic mice model (day 5, day 10, day 15 and day 21). Quantitative real-time PCR analyses of the expression Lrg1 D and ELISA analyses of media of AML12 E treated with vehicle or recombinant IL6/G-CSF/CXCL13/CCL12/TIMP1. n = 3 independent experiments. Data are means ± SD. F, G qRT-PCR and Western blot analyses of LRG1 in AML12 cells treated with cytokine combinations. n = 3 independent experiments. Data are means ± SD. H, I qRT-PCR and western blot analysis of the expression LRG1 and β-actin in AML12 cells treated with indicated serum and anti-IL6 or tocilizumab. n = 3 independent experiments. Data are means ± SD. J ELISA analyses of serum samples for IL6 from sham-group, CRC orthotopic model at day 21(PMN) and CRC intrasplenic model at day 21(Met) in BALB/c. n = 4. Data are means ± SD. K Correlation between serological IL6 and LRG1 levels of CRC patients is shown using Pearson’s correlation analysis. Dots represent individual samples. n = 161. L, M Schematic of the experimental design L . Representative images of liver metastases M in each group ( n = 6 in Lrg1 (+/+)Hep-HTVi-Ctrl group and Lrg1 (+/+)Hep-HTVi-IL6 group, n = 5 in Lrg1 (Δ/Δ)Hep-HTVi-Ctrl group and Lrg1 (Δ/Δ)Hep-HTVi-IL6 group). Scale bars, 1 cm. N Schematic of the experimental design. Representative bioluminescence images and analyses of liver metastases in CRC mouse model treated with anti-IL6 or IgG. O Schematic of the experimental design. P ELISA analyses of samples for IL6 levels from liver interstitial fluid, peripheral blood and tumor interstitial fluid of mice from different groups as indicated. n = 4. Data are means ± SD. Q ELISA analyses of samples for LRG1 levels from liver interstitial fluid and peripheral blood of mice from different groups as indicated. n = 4. Data are means ± SD. R Western blot analyses of LRG1 and β-actin in hepatocyte of mice from different groups as indicated. Statistical significance was determined using two-tailed unpaired Student’s t test A, D–F, H, and N–P

    Article Snippet: ELISA kits were used to measure the levels of human LRG1 (Ray Bio), human IL6 (Boster, EK0410), mouse LRG1 (ELK Biotechnology), and mouse IL6 (Boster, EK0411) in cell culture supernatants or serum samples according to the manufacturer’s instructions.

    Techniques: Expressing, Cell Culture, Western Blot, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Recombinant, Quantitative RT-PCR, Two Tailed Test